Determination of Selenium Speciation in High Se-Enriched Edible Fungus Ganoderma lucidum Via Sequential Extraction
نویسندگان
چکیده
Edible fungi are often used as an important selenium (Se) source to improve human daily Se intakes food or supplements because of their strong ability accumulate Se, in which Ganoderma lucidum was widely accepted China. However, the existing speciation analysis methods such protease K-based trypsin-protease XIV-based, have low extraction rate and enzymatic hydrolysis (<30%) on Se-enriched edible fungi, resulting that it is impossible effectively evaluate transformation safety fungi. In order rates, 12 (combination buffer solutions enzymes) including 4 two-step 8 three-step were applied extract from high (Total content 245.7 μg/g dry matter (DW)) present study. The results displayed one sequential method aqueous solution extraction-pepsin extraction-trypsin performed best, by total could reach 65%, enzyme 40%, revealed Selenite (63.6%), SeCys2 (20.1%), SeMeCys (14.8%) SeMet (1.5%) this lucidum. This study offers a reliable efficient
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ژورنال
عنوان ژورنال: Horticulturae
سال: 2023
ISSN: ['2311-7524']
DOI: https://doi.org/10.3390/horticulturae9020161